The objective of this course is to familiarise the student with the theory and practice of the state of the art of analytical and preparative chromatographic separation processes. Topics include: Theory of chromatography, chromatographic techniques and LC method selection and development (e.g. choice of sample preparation, columns, mobile phase and detector). An emphasis on development and optimising chromatographic methods coupled to MS will be made. Tutorials, critical reviews of the current literature and laboratory demonstrations in the lab will be employed throughout the course to illustrate important concepts and familiarise students with instrumentation.