Jordan University of Science and Technology

Intracellular nitric oxide measurement in human sperm using 4, 5-diaminofluorescein-2-diacetate and flow cytometry

Authors:  R. Sharma, S. Bani Hani, M. Bayachou, E. Sabanegh, A. Agarwal

Nitric oxide (NO) has a major contribution in male reproduction, especially in sperm motility. Methods for direct measurement of NO in single cell such as spermatozoa are complex, lack sensitivity, and are non-specific. The objective of this study was to standardize the measurement of intracellular nitric oxide in human spermatozoa using NO specific probe 4,5-diaminofluorescein-2-diacetate (DAF-2DA) and flow cytometry.