Abstract:
Neurotrophins are well-known growth factors
which can play integral roles in the control and regulation of
the gastrointestinal tract (GIT). The anabolic steroid testoster?one is involved in altering the contractile function of the stom?ach and intestine. The interactions between neurotrophin fac?tors and testosterone are strong in many systems such as the
nervous and respiratory systems. However, these interactions
are not clearly determined in the gastrointestinal tract. The aim
of this study is to investigate the effect of the anabolic steroid
sustanon on the expression levels of neurotrophins in the
chicken GIT. Neurotrophins? ELISA was done on normalized
total protein extracts from the stomach, intestine, and colon,
and the results were compared between control and sustanon?treated groups. BDNF, NGF, NT-3, and NT-4 are present at
different levels in the entire wall of the chicken GIT. Sustanon
increased BDNF levels in the stomach and intestine in dose?dependent manner and had no effect in the colon. Sustanon
had no effect on NGF in the stomach and intestine; however,
at high doses, it increased NGF levels in the colon. High dose
of sustanon increased the levels of NT-3 in all regions of the
chicken GIT. The effect on NT-4 was obviously region spe?cific, where sustanon had an inhibiting effect in the stomach,
augmenting effect in the intestine, and no effect in the colon.
These results indicate that the anabolic steroid sustanon has
differential effects on neurotrophins? levels in the chicken GIT
and this effect might explain the GIT symptoms associated
with the use of these steroids.