Jordan University of Science and Technology

louroquinolones-induced Antibacterial Activity Atteneuation by Pretreatment with Vitamin B12


Authors:  Masadeh M
Alzoubi KH
Al-Azzam S.


Abstract:  
Fluoroquinolones antibiotics action involves interfering with bacterial DNA replication and transcription leading to increased oxidative stress and bacterial cell death. Vitamin B12, on the other hand, has been shown to possess DNA protective and antioxidant properties. In this study, the possible interactive effect of Vitamin B12 on fluoroquinolones antibiotics-induced cytotoxicity against several reference bacteria was investigated. Standard bacterial strains included E. coli ATTC 35218, Staphylococcus aureus ATTC29213, Pseudomonas aeruginosa ATTC 9027, S. epidermidis ATTC 12228, A. baumannii ATTC 17978, P. mirabilis ATTC 12459 and Klebsiella pneumoniae ATTC 13883. The antibacterial activity of fluoroquinolones (ciprofloxacin and levofloxacin), with or without pretreatment of bacterial cells by vitamin B12 was assessed using the disc diffusion method and by measuring the Minimum Inhibitory Concentration (MIC) and zones of inhibition of bacterial growth. All of the tested bacterial strains were sensitive to both ciprofloxacin and levofloxacin. When pretreated with Vitamin B12, all bacterial strains showed significantly smaller zones of inhibition and larger MIC values compared ciprofloxacin or levofloxacin alone. In conclusion, results indicate the possible antagonistic properties for Vitamins B12 when it is used concurrently with flouroquinolones.