Marya Obeidat, Wisam Algargaz, Marwa Barukba, Khaldon Bodoor, Issa Mohamad, Farid Barakat, Samir Al Bashir
Abstract:
Aim: The purpose of this study was to assess the clinicopathological features
of oropharyngeal cancer patients in Jordan based on their HPV status.
Methods: Sixtynine
biopsies from two hospitals were included. Tissue microarrays were prepared fro
m formalin-fixed-paraffin-embedded (FFPE) specimens
and stained with antibodies for CDKN2A/P16, EGFR, PI3K, PTEN,AKT, pS473AKT, P
S2mTOR, and TIMAP. The cohort was divided according to P16 expression. Chisquare
and survival analyses were employed to evaluate the variations among
the study variables and determine the prognostic factors, respectively.
Results: P16 expression was found in 55.1% of patients; however, there was no
significant association between P16 expression and the patients? clinicopathological
features. Survival analysis revealed that smoking in P16-positive group and younger
age (<58 years) negatively impacted disease-free survival (DFS) (P=0.04
and P=0.003, respectively). Multivariate Cox regression test indicated that smoking,
age, PI3K, and AKT were negative predictors of
DFS (P= 0.021, P=0.002, P=0.021, P=0.009, respectively), while TIMAP was a positive
predictor (P= 0.045).
Conclusion: Elevated P16 expression is found in more than half of the patients?
specimens. DFS is negatively affected by younger age and the combined effect of
smoking and P16 overexpression. TIMAP is overexpressed in P16-positive
oropharyngeal cancer and has a favourable prognostic effect on DFS.